Figure 5: Hepatocyte GSH. HPLC runs of liver acid-soluble supernatants, showing the GS-bimane peaks with a retention time of 14.3 min. Specimens were incubated at 37°C without addition with 50 μM sorafenib or 50 μM regorafenib. At t =3 h, the specimens were transferred to the mBBr labeling reaction and processed and analyzed as described in Methods.