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Figure 3: Proliferating cell nuclear antigen (PCNA) and cleaved poly ADP ribose polymerase (cPARP) protein expression, and extracellular lactate dehydrogenase activity (LDH) after P. strombulifera treatment on HCT-116 and MCF-7 cell lines. Treatment concentrations presented are 0 μg/ml, IC50 and LC50 in both cell lines. α-tubulin was used as protein loading control. Western Blot positive control group (Co) was obtained from proteins of harvested MCF-7 culture cells. LDH results are shown as mean ± SEM of three different assays performed in triplicate. Statistical difference between treatments and control group (a, b and c) was determined using one-way ANOVA and Dunnett’s posttest for multiple comparisons, p<0.05. |