Figure 4: Clonogenic survival assay of P. strombulifera treated HCT-116 and MCF-7 cell lines. Positive colonies (≥ 30 cells) after 2 weeks are presented. HCT-116 and MCF-7 cells were previously treated at 0 μg/ml, IC50 and LC50; for 48 and 72 hrs, respectively. Clonogenicity of the control cells was considered 100%. Results are shown as mean ± SEM of three different assays performed in triplicate. Statistical difference between treatments and control group (a and b) was determined by one-way ANOVA and Dunnett’s post-test for multiple comparisons, p<0.05.