Figure 2: Phase and fluorescent micrographs of acridine orange and propidium iodide double-stained PC-3 cells. Cells were treated at IC50 values of extracts from B. ariensis from the state of Mexico and Morelos (e and f respectively), B. galeottiana (g) and B. kerberi (h) in a time-dependent manner. Cells were cultured in RPMI 1640 media maintained at 37°C and 5% CO2. Untreated cells after 72 h showed normal structures without prominent apoptosis and necrosis (a). Blebbing and orange color representing the hallmark of late apoptosis were noticed by g and h, early apoptosis features were represented by intercalated acridine orange (bright green) amongst the fragmented DNA (b, c and e-h), and red bright colored necrosis was observed by H2O2 treatment (d). Images are representatives of one of three similar experiments. Fluorescence and phase contrast images were captured at the field. Fluorescence images of samples were captured at 10x and 40x magnification.