| |
Viability (%) |
Cell Number (RFU) |
| |
Normal Media |
Cytotox. Media |
Normal Media |
Cytotox. Media |
| |
24 hr. |
48 hr. |
24 hr. |
48 hr. |
24 hr. |
48 hr. |
24 hr. |
48 hr. |
| MCF-12A |
71.96
± 2.11 |
77.49
± 0.95 |
75.83
± 1.73 |
67.08c
± 0.68 |
5040.0
± 196.1 |
6514.6a
± 320.5 |
5326.1
± 403.3 |
4993.8c
± 66.1 |
| MDA-MB-453 |
78.56
± 0.35 |
87.03a
± 0.62 |
83.52b
± 0.46 |
84.53c
± 0.34 |
15248.1
± 73.1 |
8081.3a
± 311.0 |
14881.8
± 76.4 |
9851.3c,d
± 206.3 |
| MCF-7 |
78.65
± 1.10 |
73.63a
± 0.87 |
78.66
± 0.33 |
74.77
± 1.21 |
7146.0
± 259.6 |
5341.1a
± 49.1 |
5779.3b
± 37.1 |
4113.4c,d
± 79.6 |
| Normal growth media for each cell type is defined in methods, the cytotoxicity media is DMEM without phenol red, plus 1% (low) fetal bovine serum and 4mM Glutamine.Data was analyzed by 2-way ANOVA (Media v. Time) comparison for each cell line.Post-hoc comparisons were completed using the Sidak’s test with corrections for multiple comparisons.Data are expressed as mean ± S.E. for N=4 assayed in duplicate.A significance level was established at P<0.05.
a: 24 hour normal media v. 48 hour normal media
b: 24 hour normal media v. 24 hour cytotoxicity media
c: 48 hour normal media v. 48 hour cytotoxicity media
d: 24 hour cytotoxicity media v. 48 hour cytotoxicity media |
|
