Group Dose Purpose of group Duration
Group 1 Distilled water Negative control 15 days
Group 2 1/4th LD50 EMS Positive control EMS 24 h
Group 3 Ab-EAE 100 mg/kg bw Positive control Ajugabracteosa 24 h
Group 4 Ab-EAE 400 mg/kg bw Positive control Ajugabracteosa 24 h
Group 5 Ab-EAE 100 mg/kg bw + EMS Treated Group 30 days
Group 6 Ab-EAE 200 mg/kg bw + EMS Treated Group 30 days
Group 7 Ab-EAE 300 mg/kg bw + EMS Treated Group 30 days
Group 8 Ab-EAE 400 mg/kg bw + EMS Treated Group 30 days
Table 1: Grouping, dose (distilled water, EMS and Ab-ME in concentrations of 100, 200, 300 and 400 mg/kg bw) and duration of experiment. Ab- EAE=Ethyl acetate extract of Ajuga bracteosa .