Figure 2: Effects of THC on the MBP content. Panel A shows representative western blots of MBP and β-actin. Panel B shows quantification of the MBP content expressed as percentage of untreated control cultures (=100%). Panel C shows quantification of the MBP content expressed as percentage of cultures treated with the inflammatory agents (=100%). Cultures were harvested 48 hours after the last IFN-γ plus LPS treatment. Measuring β-actin assessed equal loading of protein. Each value is the mean of 9 replicate cultures. Results were statistically evaluated for significance by the Kruskal-Wallis test followed by the Mann-Whitney test. (***P<0.001, compared with untreated control cultures; °P<0.01 compared with cultures treated with the inflammatory agents).