Figure 6: A. Effects of THC and of the PPAR-γ antagonist GW9662 on IFN-γ + LPSinduced mRNA expression of i-NOS Aggregate cultures remained either untreated or were treated with THC (1 mM) or GW9662 (2 mM). GW9662 and THC were applied 1 hour before IFN-γ (50 U/ml) plus LPS (5 mg/ml). These treatments were applied once (1x) or three times (3x). For measuring the mRNA expression, cultures were harvested 24 hours after the inflammatory treatments. Values are expressed as fold change of untreated control cultures (=1), each value being the mean of 8 replicate cultures. Results were statistically evaluated for significance by the Kruskal- Wallis test followed by the Mann-Whitney test. (*P<0.05, **P< 0.01, ***P<0.001 compared with untreated control cultures; oooP< 0.001 compared with to cultures treated with the inflammatory agents). B. Effects of THC on PPAR- γ mRNA expression Aggregate cultures remained either untreated or were treated with THC (1 mM) or GW9662 (2 mM). GW9662 and THC were applied 1 hour before IFN-γ (50 U/ml) plus LPS (5 mg/ml). These treatments were applied once (1x) or three times (3x). For measuring the mRNA expression, cultures were harvested 24 hours after the inflammatory treatments. Values are expressed as fold change of untreated control cultures (=1), each value being the mean of 5 replicate cultures. Results were statistically evaluated for significance by the Kruskal- Wallis test followed by the Mann-Whitney test. (*P<0.05, **P< 0.01, compared with untreated control cultures; oP< 0.05 compared to cultures treated with the inflammatory agents).