Additions

ADP

DNP

RCR

 

V3

V4

 

 

control

150 ± 9

40 ± 8

240 ± 15

3.7 ± 0.2

buterol

 

 

 

 

100 µM

150 ± 11

48 ± 9

240 ± 15

3.1 ± 0.4

200 µM

136 ± 9

54 ± 8

244 ± 17

2.5 ± 0.3*

500 µM

130 ± 7

56 ± 9

240 ± 17

2.3 ± 0.2*

NEM

 

 

 

 

50 µM

151 ± 9

49 ± 9

248 ± 14

3.1 ± 0.5

100 µM

117 ± 12

51 ± 11

240 ± 18

2.3 ± 0.6*

200 µM

85 ± 11

46 ± 7

240 ± 16

1.8 ± 0.3*

500 µM

52 ± 9

34 ± 8

220 ± 18

1.5 ± 0.4*

Respiration rates in the presence of 0.2 mM ADP (V3 and V4) and 50 μM DNP are presented. Substrate of oxidation was 4 mM succinate in the presence of 1 μM rotenone. RCR is the respiratory control ratio. * Statistical significance p < 0.05.
Table 1: Effect of buterol and NEM on the respiration of mitochondria