Placebo Alcohol Two-way ANOVA P value
Parameters 0% GTP (P) 0.1% GTP (PLG) 0.5% GTP (PHG) 0% GTP (A) 0.1% GTP (ALG) 0.5% GTP (AHG) Alcohol GTP Alcohol GTP
BV/TV (%) 8.41 ± 0.49x 7.51 ± 0.49x 8.41 ± 0.46x 5.98 ± 0.52y 5.83 ± 0.49y 5.35 ± 0.46y <0.001 0.572 0.356
Tb.N (1/mm3) 2.356 ± 0.141x 2.087 ± 0.141x    2.368 ± 0.133x 1.743 ± 0.151y 1.509 ± 0.141y    1.521 ± 0.133y  <0.001 0.222 0.567
Tb.Th (mm) 0.056 ± 0.001x 0.055 ± 0.001x    0.055 ± 0.001x 0.052 ± 0.001y 0.054 ± 0.001y    0.052 ± 0.001y  0.004    0.524 0.417
Tb.Sp (mm) 0.421 ± 0.041y 0.468 ± 0.041y    0.427 ± 0.039y 0.686 ± 0.045x 0.660 ± 0.041x    0.669 ± 0.039x  <0.001 0.921 0.678
Conn Dens (1/mm) 31.82 ± 3.05x 25.21 ± 2.85x 31.49 ± 2.69x 21.89 ± 3.05y 21.82 ± 0.30y 16.95 ± 2.69y <0.001 0.508 0.156
SMI 2.55 ± 0.05y 2.60 ± 0.05y 2.53 ± 0.04y 2.76 ± 0.05x 2.73 ± 0.03x 2.82 ± 0.04x <0.001 0.916 0.240
Alcohol negatively affected all parameters of trabecular bone in the Tibia
Table 3: Trabecular bone parameters of the tibia measured in placebo - and alcohol-treated rats supplemented with green tea polyphenols (GTP) in drinking water are shown. Results are expressed as mean values ± standard error of mean (SEM). All dietary treatment groups were analyzed by two-way analysis of variance (ANOVA) followed by Fisher’s protected least significant difference (Fisher’s LSD) post-hoc test to evaluate the effect of alcohol, GTP dose, or interaction. Within a row with different superscripts (x and y for alcohol effect; a and b for GTP; A, B and C for interaction) are significantly different by two-way ANOVA and Fisher’s LSD test (P<0.05). Max force, maximal force. Abbreviations are: BV/TV, bone volume/total volume; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation; Conn Dens, connectivity density; SMI, structural model index.