Placebo Alcohol Two-way ANOVA P value
Parameters 0% GTP (P) 0.1% GTP (PLG) 0.5% GTP (PHG) 0% GTP(A) 0.1% GTP (ALG) 0.5% GTP (AHG) Alcohol GTP Alcohol GTP
BV/TV (%) 0.967 ± 0.001 0.968 ± 0.001 0.969 ± 0.001 0.969 ± 0.001 0.966 ± 0.001 0.967 ± 0.001 0.640 0.952 0.304
Cortical thickness (mm) 0.521 ± 0.009xb 0.550 ± 0.008xa 0.545 ± 0.008xa 0.474 ± 0.009yb 0.482 ± 0.008ya 0.494 ± 0.008ya <0.001 0.032 0.443
Cortical Area (mm2) 9.090 ± 0.223x 9.608 ± 0.223x 9.414 ± 0.210x 7.848 ± 0.258y 7.894 ± 0.223y 8.480 ± 0.238y <0.001 0.168 0.276
Medullary Area (mm2) 0.118 ± 0.005x 0.118 ± 0.005x 0.115 ± 0.004x 0.093 ± 0.005y 0.102 ± 0.005y 0.107 ± 0.005y <0.001 0.528 0.301
Porosity (%) 3.234 ± 0.123 3.096 ± 0.103 3.190 ± 0.115 3.085 ± 0.133 3.426 ± 0.123 3.538 ± 0.133 0.092 0.280 0.097
Alcohol negatively affected tibial mid-diaphysis parameters.
Table 4: Parameters of tibial mid-diaphysis in placebo- and alcohol-treated rats supplemented with green tea polyphenols (GTP) in drinking water are shown. Results are expressed as mean values ± standard error of mean (SEM). All dietary treatment groups were analyzed by two-way analysis of variance (ANOVA) followed by Fisher’s protected least significant difference (Fisher’s LSD) post-hoc test to evaluate the effect of alcohol, GTP dose, or interaction. Within a row with different superscripts (x and y for alcohol effect; a and b for GTP; A, B and C for interaction) are significantly different by two-way ANOVA and Fisher’s LSD test (P<0.05). Abbreviations are: BV/TV, bone volume/total volume.