| Factor |
Level used [Coded] |
| Independent Variables |
Low [-1] |
Medium [0] |
High [+1] |
| X1= Lipid: drug [w/w] |
21.36 |
26.70 |
32.40 |
| X2= Lipid: Surfactant [w/w] |
4.50 |
5.00 |
5.50 |
| X3= Sonication time [min] |
10.00 |
20.00 |
30.00 |
| Dependent Variables =Y1 [Entrapment Efficiency [%]], Y2 [Particle size [nm]] and Y3 [Transdermal flux [μg/cm2/hr]] |
| Design Results |
| Code |
X1 [w/w] |
X2 [w/w] |
X3 [min.] |
Y1 [%] mean ± SD, n=3 |
Y2 [nm] mean ± SD, n=3 |
Y3 [ug/cm2/hr] mean ± SD, n=3 |
| GNTs1 |
0 |
0 |
0 |
69.10 ± 0.74 |
145.24 ± 3.48 |
53.39 ± 4.76 |
| GNTs2 |
0 |
0 |
0 |
68.80 ± 0.56 |
142.24 ± 1.89 |
54.10 ± 3.63 |
| GNTs3 |
+1 |
+1 |
0 |
59.96 ± 1.21 |
180.06 ± 5.56 |
36.65 ± 5.30 |
| GNTs4 |
-1 |
0 |
+1 |
48.95 ± 1.23 |
156.15 ± 4.98 |
40.10 ± 3.81 |
| GNTs5 |
0 |
0 |
0 |
68.90 ± 1.67 |
152.76 ± 3.80 |
53.40 ± 4.76 |
| GNTs6 |
+1 |
0 |
+1 |
51.18 ± 2.56 |
166.58 ± 1.24 |
46.04 ± 2.48 |
| GNTs7 |
0 |
+1 |
-1 |
86.88 ± 1.33 |
184.67 ± 3.43 |
42.78 ± 5.44 |
| GNTs8 |
-1 |
0 |
-1 |
50.40 ± 0.75 |
158.32 ± 5.60 |
47.30 ± 7.20 |
| GNTs9 |
0 |
-1 |
+1 |
72.68 ± 2.23 |
151.33 ± 4.69 |
48.52 ± 5.09 |
| GNTs10 |
+1 |
-1 |
0 |
42.60 ± 1.32 |
140.04 ± 2.35 |
41.57 ± 9.36 |
| GNTs11 |
0 |
0 |
0 |
69.10 ± 0.98 |
142.31 ± 1.36 |
53.39 ± 4.76 |
| GNTs12 |
0 |
0 |
0 |
68.80 ± 3.34 |
147.68 ± 3.50 |
54.10 ± 3.63 |
| GNTs13 |
+1 |
0 |
-1 |
51.17 ± 2.35 |
170.24 ± 4.21 |
36.56 ± 5.49 |
| GNTs14 |
0 |
-1 |
-1 |
74.61 ± 1.13 |
175.09 ± 1.25 |
41.40 ± 3.21 |
| GNTs15 |
-1 |
+1 |
0 |
54.33 ± 0.87 |
160.78 ± 2.12 |
39.94 ± 4.27 |
| GNTs16 |
0 |
+1 |
+1 |
86.20 ± 4.39 |
191.23 ± 3.22 |
38.31 ± 5.04 |
| GNTs17 |
-1 |
-1 |
0 |
45.59 ± 0.87 |
154.33 ± 3.89 |
43.50 ± 4.58 |
Y1=entrapment efficiency (%); Y2=particle size (nm) and Y3=transdermal flux μg/cm2/hr) |
