Figure 2: Light, transmission and scanning microscopy of epimastigotes. (a) Untreated epimastigotes stained with Giemsa showed typical morphology by light microscopy. (b) Normal body ultrastructure when observed by scanning electron microscopy. (c) Epimastigotes treated with 1 mM of compound 1, for 24 h showed loss of cytoplasmic volume and condensation of the parasite. However, normal parasites could also be observed. (d) The loss of cytoplasmic volume and condensation of the parasite demonstrated by scanning electron microscopy. (e) An extracellular parasite with organelles such as the Golgi complex and mitochondria morphologicaly preserved. (f) white arrowhead showed the extracellular features of epimastigotes treated 1mM for 48 h, where unaltered cytoplasm retraction of the parasite (star) with plasmatic membrane blebs formation occurred. Scale bars: A=15 μm; B, D, E, F=0.5 μm; C=10 μm;