% infected cell  treated for 24h            Mean Number of intracellular parasites Compound Control 0.1mM 1mM 5mM 10mM Control 0.1mM 1mM 5mM 10mM 1 84 ± 12 69 ± 6 37 ± 5 11 ± 5 tox 5063 ± 37 3566 ± 56 1965 ± 45 530 ± 23 tox 2 60 ± 6 62 ± 4 46 ± 7 13 ± 5 tox 999 ± 22 785 ± 124 886 ± 34 560 ± 17 tox 3 22 ± 1 14 ± 2 6 ± 1 5 ± 2 tox 335 ± 13 221 ± 21 67 ± 7 55 ± 14 tox 4 67 ± 3 71 ± 5 46 ± 4 12 ± 4 tox 944 ± 84 846 ± 75 436 ± 91 220 ± 35 tox 5 30 ± 1 25 ± 1 15 ± 1 12 ± 1 tox 981 ± 51 811 ± 19 580 ± 38 215 ± 19 tox 6 30 ± 2 25 ± 1 20 ± 1 14 ± 1 tox 655 ± 28 580 ± 13 422 ± 16 333 ± 13 tox 7 31 ± 1 26 ± 0.9 20 ± 1.5 16 ± 1 tox 844 ± 20 577 ± 15 298 ± 16 214 ± 8 tox 8 41 ± 3 29 ± 3 19.0 ± 2 12 ± 2 tox 900 ± 45 621 ± 31 259 ± 12 133 ± 9.2 tox 9 32 ± 1 26 ± 0.9 20 ± 1.5 16± 1 tox 844 ± 20 577 ± 15 298 ± 16 214 ± 8 tox 10 34 ± 2 27 ± 1 24 ± 2 0 tox 730 ± 48 586 ± 24 481 ± 45 0 tox 11 40 ± 3 29 ± 3 19 ± 2 12 ± 2 tox 900 ± 45 620 ± 81 259 ±11 133 ± 9 tox 12 31 ± 1 23 ± 1 18 ± 2 17 ± 1 tox 690 ± 34 477 ± 35 257 ± 18 222 ± 7 tox 13 81 ± 7 59 ± 8 20 22 tox 830 ± 58 785 ± 48 316 ±25 102 ± 20 tox 14 81 ± 8 59 ± 6 18 tox tox 840 ± 56 774 ± 60 337 ± 28 tox tox BZN 39 ± 1 32 ± 1 22 ± 1 13 ± 5 8 ± 2 980 ± 39 762 ± 38 487 ± 48 257 ± 11 137 ± 8 HU 88 ± 1 82 ± 1 76 ± 2 14 ± 2 0 1265 ± 13 959 ± 18 759 ± 22 173 ± 39 0

Table 3: Cultures of Vero cells infected with T. cruzi for 48 h and treated with compounds (1–14), HU and BZN for different concentrations. Effects of compounds on the infection and intracellular proliferation of parasite were observed. Values are mean ± SD (n=3).