Patch Number of Tissues   Time (hr)   Compound/Metabolite( ng/mL ± SD)a
  Donepezil   M1/M2   M6   M4   M3
  Test Article   3   1 371.6±586.7 ND ND ND NA
  4 619.1±678.2 2.1±3.7 8.8±15.3 10.7±18.6 NA
  8 1038.1±555.1 5.9±5.4 14.9±22.5 20.1±33.0 NA
  24 1720.4±578.1 22.6±25.0 22.0±29.7 42.9±57.4 NA
  48 1860.3±459.0 17.0±12.2 53.1±26.4 114.5±91.7 NA
Note: Mean concentrations of donepezil and its metabolites were estimated using a single point calibration. ND, Not Detected; NA, detected, quantitative data not available; aNomenclature was taken from Matsui et al. (Matsui K, Mishima M, Nagai Y, Yuzariha T, and Yoshimura T (1999) Drug Metab Dispos 27: 1406- 1414)
Table 2: Assessment of Levels (ng/mL) of Donepezil and Its Metabolites in Reconstituted Human Epidermis by LC/MS/MS.