Fractions % of TRR
SPE fraction 1a - Non retained material 0.46
SPE fraction 2a - 1% acetic acid in water 0.54
SPE fraction 3a - 1% acetic acid in water:acetonitrile (95:5, v/v) 0.51
SPE fraction 4a - 1% acetic acid in water:acetonitrile (90:10, v/v) 1.37
SPE fraction 5a - 1% acetic acid in water:acetonitrile (75:25, v/v) 15.48
SPE fraction 6a - 1% acetic acid in water:acetonitrile (50:50, v/v) 57.68
SPE fraction 7a - 1% acetic acid in acetonitrile 14.36
Total recovery 90.36
TRR – Total radioactive residue
Table 1: The Extraction and Distribution of Radioactivity from Enzyme Hydrolysed
Urine Using Solid Phase Extraction.