Figure 6: Effect of compound C on AMPK and ERK activation. Differentiated C2C12 myotubes were serum starved for 2 h and treated with 50 μM Compound C (CC) for 1 hour followed by treatment with 100 nM EA in the presence CC for 30 minutes. EA induced phosphorylation of AMPK (A) and ERK 1/2 (B) were reduced by CC treatment. Images shown are representative of three independent experiments and the quantitative data are average of three experiments. *P<0.05 vs. unstimulated cells. #P<0.05 vs. EA stimulated cells.