Set 1-Primer assay for SNP conformation

Gene Forward Reverse Product length Tm CBLN2 TGTCTTTTCCAAGGGGATGA TTTGATGCATAACAGGCAACT 178 60 TTLL7 TGTAAGGTGGAGGCTTAGAGAAA ACACTCATGCACACACACACA 310 60 WDR40C (DCAF12L2) GTGGGAAGCAGTGGTAGGG AGCCCCAAAGTGGGTTTTAC 226 60 ATP2B3 AAGTGGTTGAGTGGGTGTCC AGAAGCCCCAAATGGACTTT 280 60 RAB39B CAAGGTCATTTTGGGGTGAG GGACAAAGGTAAAGCCCATTC 205 60

Set 2-Primer assay for qRT-PCR

Gene Forward Reverse Product length Tm CBLN2 GCAAGTGCTTGGTGGTGTG AAATCCCTTTTCTCGGTGCT 243 60 TTLL7 ATGATGGACTTGTGCGAATG ACGTTTGCTGCCTTTGTTCT 152 60 WDR40C (DCAF12L2) CTGCGATGAGTTGTCCCTCT GGCTCTCTCCTCCAGGAACT 220 60 ATP2B3 TGACCTCACCTGCATAGCTG GGTTGAACTCCTTCCCTTCC 206 60

Set 3-Primer assay for coding variants

Gene Forward Reverse Product length Tm CBLN2         Exon 1 AACGACCCTGGCCTTGAC GAACTCCACGTCCAAGAAGC 596 60 Exon 2 ACTGAGGAGGGGCTTGCTAT CCAAACCTGAAAGGATCCAG 205 60 Exon 3 TGCTGAGCTCTACTCTCTGCAT CCACCATCTAGGGTGCTCTG 248 60

Table 4: Primer assays for 5 candidate genes in rhesus.