Figure 4: Effects of Nifedipine (N) on S43126(S)-induced insulin release. Voltage-gated L-type calcium channel blocker nifedipine [10 μM] attenuated the insulinotropic response of Min6 cells to S43126 under (3A) low and (3B) high glucose. (3A) Data are presented as mean percent change +/- standard error from three separate experiments run in duplicate. (a=*p< 0.05, ***p< 0.001 same time point, compared to N and b= **p< 0.01, ***p< 0.001 same dose, different time compared to 5 mins, ANOVA). (3B) Data are presented as mean percent change +/- standard error from three separate experiments run in duplicate. (a= ***p< 0.001 same time point, compared to N and b= **p< 0.01, ***p< 0.001 same dose, different time compared to 5 mins, twoway ANOVA).