Figure 7: Dose-Response WB. Effects of S43126 [10-7 10-5M] on (A) IRAS protein expression (B) ERK1/2 phosphorylation (C) PKB (Akt) phosphorylation. S43126 [10-7 10-5M] caused an increased induction of IRAS protein, and a dose-dependent increase in the phosphorylation of both ERK1/2 and PKB. The relative protein expression of IRAS was defined by the ratio of the β-actin while the relative phosphorylation of ERK1/2 and PKB was defined by the ratio of phosphorylated active form to total immunoreactive protein, in arbitrary absorbance units. Data are presented as mean percent change +/- standard error from two separate experiments run in duplicate. (***p< 0.001 Control vs Dose [10-7-10-5M], one-way ANOVA).