Figure 5: Cytokine mediated β-cell death was attenuated by SIRT1 knockdown and was potentiated by SIRT1 overexpression MIN6 cells were exposed as indicated to cytokines for 6 hrs at high density. Cell survival was assayed by 0.1% MTT staining. In SIRT1 overexpressing MIN6 cells, 2x naphthaldehyde (60 micromol/L) was required for efficacy. # signifies difference from cytokine exposure alone; $ signifies difference from similarly exposed control cultures at P < 0.05.