◄▪▪►Hypertrophy of cell ▬►Condensation of chromatin after colored with MGG ▬►after colored with acridine orange ★Fragmentation of nucleoli ★Vacuolization of cytoplasm

Figure 7: MGG staining of P. obesus aortic fibroblasts, exposed at 10 UI/ml of insulin during 6h. (A)Control aortic fibroblasts P. obesus, (B and C) Aortic fibroblasts P. obesuse submitted at 10 UI/ml of insulin during 6h, fixed in aqueous Bouin and stained with May Grunwald Giemsa (MGG), the observation was done with an inverted microscope (Zeiss) (GX200), (D) Control aortic fibroblasts of P. obesus, (E) Aortic fibroblasts P. obesuse submitted at 10 UI/ml of insulin during 6h, fixed in aqueous Bouin and stained with acridine orange, the observation was done with a fluorescence microscope ( by green filter), (F and G) Aortic fibroblasts P. obesuse submitted at 10 UI/ml of insulin during 6h, fixed in aqueous Bouin and stained with acridine orange The observation was done with a fluorescence microscope ( by blue filter) (GX200).