****p < 0.0001 vs control
Figure 12: Separation of a1 (I+III) and a 2 (I) collagen chains contained in the extracellular compartment of cultured aortic fibroblasts control and aortic fibroblasts control exposed at 10 UI/ml of insulin during 6h of P. obesus. The a chains were analyzed after reduction with 0.25% -mercaptoethanol by electrophoresis on 10% SDS polyacrylamide gel. Gels were stained with cooamassie blue and discolored in 10% acetic acid