Figure 3: Effect of calcitriol on KLF4, RANK, TXNIP NFκB (p50/p65) protein expression in VSMC stimulated with diabetic environment. Western blots (A) and their densitometric analysis (B,C) of KLF4, RANK, NFκB (p50/p65), and TXNIP measured in VSMC after incubation for 24 h with HSA (200 μg/ml), AGE-HSA (200 μg/ml), glucose (250 mg/dl) and calcitriol (10-9 mol/l and 10-10 mol/l). The membrane was stripped and reprobed with α-tubulin which is shown as the loading control. Data are expressed as mean ± SD of 4-5 independent experiments. *p<0. 05 compared to control group-HSA; #p<0. 05 compared with AGE-Glucose.