Figure 4: Overexpression of adiponectin prevented synergic effect of resistin and TNF-α in 3T3-L1 adipocytes. 3T3-L1 adipocytes were transduced with Ad-resistin or Ad-lacZ, and Ad-adipo. At 48 hrs after infection, serum-starved 3T3-L1 adipocytes were treated without or with TNF-α (10 ng/ mL) for 3 h. These 3T3-L1 adipocytes were treated with insulin (100 ng/mL), followed by measurement of 2-DOG uptake. Data represent the mean ± SE of 3 independent experiments (each experiment performed with 6 samples). *P < 0.05, **P < 0.01.