Figure 5: Reduced adiponectin secretion by treatment with TNF-α is rescued by infection of 3T3-L1 adipocytes with Ad-adipo. 3T3-L1 adipocytes were transduced with Ad-resistin or Ad-lacZ and Ad-adipo. At 48 hrs after infection, 3T3-L1 adipocytes were starved for 3 hrs prior to treatment without or with TNF-α (10 ng/mL) for 3 h. Conditioned medium was collected, fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotted using a polyclonal antibody against mouse adiponectin to determine the levels of secreted adiponectin. Relative levels of adiponectin released from adipocytes treated with TNF-α, as assessed from 4 independent experiments. **P < 0.01.