Figure 1: (A) Glucose tolerance in alloxan-recovered rabbits treated with tolbutamide. Alloxan-recovered rabbits, after an overnight fasting, were orally administered tolbutamide (1 g/kg bdwt). Glucose (3 g/kg bdwt) was administered orally 90 min later. Blood samples were drawn from the marginal ear vein at various intervals as shown and serum glucose concentration was determined by a glucose-oxidase method.
(B) Glucose tolerance in alloxan-recovered rabbits treated with the bark extract (silica gel chromatographic fraction) of Ficus bengalensis. Alloxan-recovered rabbits, after an overnight fasting, were orally administered with the bark extract (1 g/kg bdwt). Glucose (3 g/kg bdwt) was administered orally 90 min later. Blood samples were drawn and analyzed for serum glucose as described in A.
(C)
Insulin content of the culture media in which pancreatic beta cells were exposed to: a: none (control); b: DMSO (10 μL/mL); c: glybenclamide (1 mg/mL); and d: fenugreek protodioscin enriched fraction (FPEF; 1 mg/mL). Pancreatic beta cells were grown in 12-well cluster dishes to 60% confluence and then treated with various agents (a-d) in high glucose DMEM medium for 30 min. The insulin content of the medium was determined using an ELISA kit from Crystal Chem (Downers Grove, IL). Data points in A-C represent values of mean ± SEM (A and B) or mean ± SD (n=3; C). In Figure 1C, *p<0.01 and **p<0.05 for DMSO vs. glybenclamideand FPEF treatments (respectively). With regard to (a) untreated controls, the treatments with (c) glybenclamide and (d) FPEF are significant at p<0.05.