Figure 5: Effects of chronic prenatal ethanol exposure on Notch signaling in juvenilecerebella. Probe-based qPCR amplification reactions were used to measure (A) Notch 1, (B) Jagged 1, (C) HES 1, (D) AAH, (E) HIF-1α, and (F) FIH gene expression with results normalized to β-actin, which was measured simultaneously. Inter-group comparisons were made using the calculated mRNA/β-actin ratios. N=12/group with each sample contributed from a different litter.