| Enzyme |
Gene accession numbers
from ToxoDataBase a |
Localization |
| Citrate Synthase I (CS1) |
59.m03414 |
Mitochondrion |
| Citrate Synthase II (CS2) |
20.m03767 |
|
| Citrate Synthase III (CS3) |
42.m03311 |
|
| Aconitase (ACN) |
42.m03524 |
Mitochondrion |
| Isocitrate-Dehydrogenase I (IDH1) |
583.moo674 |
Mitochondrion |
| Isocitrate-Dehydrogenase II (IDH2) |
57.m00028 |
|
| a-Ketoglutarate-Dehydrogenase E1 (OGDH E1) |
49.m03397 |
|
| Dihydrolipoly-Transacetylase (OGDH E2) |
38.m00017 |
|
| Dihydrolipoly-Transacetylase (OGDH E3) |
20.m03954 |
|
| Succinyl-coa-synthetase alpha (Scsa) |
80.m00087 |
Mitochondrion |
| Succinyl-coa-synthetase (ATP) (Scsb) |
583.m00592 |
Mitochondrion |
| Fumarase (FUM) |
57.m01846 |
|
| Malate-Dehydrogenase (MDH) |
641.m00168 |
Mitochondrion |
| FAD Malate-Dehydrogenase (MDH-FAD) |
80.m00006 |
Mitochondrion |
| Branched-Chain a-Ketoglutarate-Dehydrogenase E1 (BCOGDH-E1) |
49.m00028 |
|
It seems that tachyzoites, but not bradyzoites, posses a functional tricarboxylic acid cycle because in cell homogenates of tachyzoites, activities of Succinate Dehydrogenase
(SDH) and NADP+-dependent isocitrate dehydrogenase were detected, while no SDH activity could be found in bradyzoites [365,372] 