Enzymes |
Bradyzoite |
Tachyzoite |
Actin |
↑ |
↑ |
UDP-glucose phosphorylase |
↑ |
↑ |
Starch (glycogen) synthase |
↑ |
↑ |
Branching enzyme 1 |
↑↑ |
↑ |
Branching enzyme 2 |
↑ |
↑↑ |
Isoamylase |
↑ |
↑↑ |
D-enzyme (α-1,4-glucanotransferase) |
↑ |
↑ |
α-glucan |
↑ |
0 |
α-amylase |
↑ |
0 |
α-glucosidase |
↑↑ |
0 |
Glycogenin |
↑ |
↑ |
R1 protein
(α-glucan water dikinase) |
↑ |
0 |
Debranching enzyme |
↑ |
↑ |
↑↑: Markedly increased gene expression; ↑: Increased expression; ↑: Weak
expression; 0: No gene present; T. gondii enzymes were identified at the
genome Web site: htttp://www.toxodb.org. The parasite genome encodes
two fructose 1,6-biphosphatase isoenzymes, a single pyruvate-carboxylase,
and two PEP-carboxykinases. The conversion from glucose-6-phosphate into
glucose-1-phosphate,which forms the link between amylopectin metabolism and
gluconeogenesis, is catalysed by two isoforms of glucosephosphate-mutase [365].
The following soluble tachyzoite antigenic proteins have been identified: a putative
protein disulfide isomerase, Hsp60, Hsp70, a pyruvate kinase, a putative glutamate
dehydrogenase, a coronin, a protein kinase C receptor 1, a malate dehydrogenase,
a major surface antigen 1, an uridine phosphorylase, and a peroxiredoxin [93] |