Table 33

Technique	Group of animals	Density of neurons
HU	Controls (n=5)	1472.80 ± 179.14
	Diabetics (n=5)	1198.80 ± 237.24^a
	Insulin-treated rats (n=5)	1181.60 ± 179.53^a
NADH	Controls (n=5)	631.80 ± 5.18
	Diabetics (n=4)	820.0 ± 8.13^a
	Insulin-treated rats (n=4)	987.5 ± 19.69^a
NOS	Controls (n=5)	454.80 ± 59.41
	Diabetics (n=5)	415.60 ± 109.30
	Insulin-treated rats (n=5)	421.60 ± 48.22

Results are expressed as mean ± SD; ^ap<0.001. HuC/HuD,anti-human neuronal protein HuC/HuD identifies all neuronal cell bodies in the ganglion; NADHd, diaphorase positive

Table 33: Density of neurons (neurons/11.07 mm²) reactive to the HuC/HuD and nNOS immunohistochemical techniques and NADHd histochemistry of the myenteric plexus of the duodenum in adult rats with experimental acute diabetes (according to de Mello et al. [495]; with own modification).