| Cell type |
Cell line |
IC50 (mg/ml)a |
|
|
|
|
|
| |
|
Heparin |
CSC |
CSA |
Dextran sulfated |
Dextran |
Fucoidine |
| Endothelial |
HUV-EC-Cb |
1.8 ± 0.2 |
1.6 ± 0.4 |
2.9 ± 1.5 |
1.0 ± 0.4 |
9.7 ± 3.4 |
4.1 ± 1.8 |
| Epithelial |
HEp-2b |
5.5 ± 1.6 |
4.5 ± 0.4 |
6.3 ± 2.3 |
5.7 ± 2.3 |
14.1 ± 2.1 |
19.5 ± 0.2 |
| Fibroblast |
HFF |
4.8 ± 2.0 |
4.1 ± 1.4 |
7.3 ± 1.7 |
2.2 ± 0.4 |
> 20 |
> 20 |
| Glial or astrocyte |
U373b |
3.0 ± 1.9 |
3.0 ± 0.8 |
1.1 ± 0.4 |
2.1 ± 0.4 |
7.4 ± 3.8 |
16.0 ± 3.7 |
| Macrophage |
U937b |
2.0 ± 0.3 |
1.8 ± 0.8 |
2.6 ± 1.5 |
6.7 ± 2.6 |
7.0 ± 2.7 |
13.2 ± 4.9 |
| Melanocyte |
G361b |
4.0 ± 0.3 |
2.0 ± 0.1 |
3.0 ± 1.9 |
4.6 ± 0.4 |
8.8 ± 4.7 |
0.3 ± 0.1 |
| Averagec |
|
3.5 ± 1.4 |
2.8 ± 1.1 |
3.9 ± 2.2 |
3.7 ± 2.1 |
11.2 ± 4.6 |
11.7 ± 6.2 |
aMean ± SE, n = 2 or 3; bObtained from the American Type Culture collection; cAverage values (± SE) for all cell types tested. dA sulfated L-fucose oligosaccharide. eThe
synthetic polyanion. IC50: Inhibitory Concentration; CSC: Chondroitin sulfate C; CSA: Chondroitin Sulfate A; Several heparin binding proteins were identified in lysates of T.
gondii based on their ability to agglutinate red blood cells in a heparin-sensitive manner [460]. |
