| SNP (rs#) |
Primers |
Amplicon
(bp) |
Annealing temp.
(°C)/[MgCl2]
(mmol) |
Restriction enzyme/allele size |
| T-786C
(rs 2070744) promoter |
P1: 5’-GCA TGC ACT CTG GCC TGA AGT G-3’
P2: 5’-CAG GAA GCT GCC TTC CAG TGC-3’ |
223 |
61.5/2 |
MspI (Bioron)/T=162, 61 bp
C=116, 61, 46 bp |
| 27 VNTR Intron 4
|
P1: 5’-AGG CCC TAT GGT AGT GCC TTT-3’
P2: 5’-TCT CTT AGT GCT GTG GTC AC-3’ |
393/420 |
59.2/3.5 |
a allele=393 bp
b allele=420 bp |
| G894T
(rs 1799983) Exon 7
|
P1: 5’-AAG GCA GGA GAC AGT GGA TGG A-3’
P2: 5’-CCC AGT CAA TCC CTT TGG TGC TCA-3’ |
457 |
62.9/3 |
BanII (New England Biolabs Inc.)
G=137, 320 bp
T=457 bp |
