Table 1

Gene	Approved symbol	Gene Bank accession number		Primer sequence	Tm (°C)	No. cycles	Product length (bp)
EGFR	EGFR	NM_005228.3	sense	CAGCAGAGACCCACACTACCAG	58	35	213
			antisense	GAGCCCTTAAAGATGCCATTTG
Akt	AKT1	NM_005163.2	sense	ACAAGGACGGGCACATTAAGAT	62	35	189
			antisense	GACCGCACATCATCTCGTACAT
PI3K	PIK3CA	NM_006218.2	sense	TGGTGAAAGACGATGGACAACT	54	35	151
			antisense	GTGCATTCTTGGGCTCCTTTAC
PTEN	PTEN	NM_000314.4	sense	CAATCCTCAGTTTGTGGTCTGC	54	35	226
			antisense	GGTTTCCTCTGGTCCTGGTATG
mTOR	MTOR	NM_004958.3	sense	GGGACTGCTTTGAGGTTGCTAT	56	35	249
			antisense	ATCGCTTGTTGCCTTTGGTATT
Cyclin D1	CCND1	NM_053056.2	sense	CCCCAACAACTTCCTGTCCTAC	60	35	186
			antisense	ACCTCCTCCTCCTCCTCTTCCT
CDK4	CDK4	NM_000075.3	sense	CGAAACGATCAAGGATCTGATG	55	35	188
			antisense	TAACAACCACGGGTGTAAGTGC
PCNA	PCNA	NM_002592.2	sense	TCAGCCATATTGGAGATGCTGT	55	35	228
			antisense	GTGTCACCGTTGAAGAGAGTGG
RB	RB1	NM_000321.2	sense	CCCCTACCTTGTCACCAATACC	62	35	246
			antisense	CACGGTCGCTGTTACATACCAT
E2F1	E2F1	NM_005225.2	sense	CGTGGACTCTTCGGAGAACTTT	58	35	187
			antisense	AGATGATGGTGGTGGTGACACT
c-myc	MYC	NM_002467.4	sense	TCCACACATCAGCACAACTACG	58	35	171
			antisense	GTGTGTTCGCCTCTTGACATTC
p53	TP53	NM_000546.4	sense	TTGGCTCTGACTGTACCACCAT	56	35	203
			antisense	TTTCTTGCGGAGATTCTCTTCC
Collagen I	COL1A1	NM_000088.3	sense	CCAGAACATCACCTACCACTGC	60	35	218
			antisense	GGGAGGTCTTGGTGGTTTTGTA
Collagen III	COL3A1	NM_000090.3	sense	TGCAAATTGGATGCTATCAAGG	56	35	205
			antisense	GCACATCAAGGACATCTTCAGG
TGF β1	TGFB1	NM_000660.4	sense	ACAATTCCTGGCGATACCTCAG	58	35	196
			antisense	GTAGTGAACCCGTTGATGTCCA
ILK	ILK	U40282.1	sense	GCATGGCTGATGTCAAGTTCTC	56	35	156
			antisense	GTGTCACCAGTTCCCACAGAAG
Glut1	SLC2A1	NM_006516.2	sense	ACCCTGGATGTCCTATCTGAGC	58	35	360
			antisense	TCCTCGGGTGTCTTGTCACTT
Insulin Receptor	INSR	M10051.1	sense	TGTCATGGATGGAGGGTATCTG	56	35	174
			antisense	CTGTGGAAGAACGACACCTCTG
Hexokinase I	HK1	NM_000188.2	sense	CCTAAATGCTGGGAAACAAAGG	58	35	193
			antisense	CAGTGCTAATCGGTCACTCTCG
Hexokinase II	HK2	NM_000189.4	sense	ACCAAGCGTGGACTACTCTTCC	58	35	156
			antisense	ACAGGTGCTCTCAAGCCCTAAG
PFK	PFKP	NM_002627.4	sense	TGGAGTGGATCACTGCAAAACT	54	35	165
			antisense	GTTCTTTGGGAATCCTGTGCTC
PKM1	PKM	NM_182470.2	sense	GAGCCTCAAGTCACTCCACAGA	58	35	227
			antisense	CTTGCACAGCACAGGGAAGAT
PKM2	PKM	NM_002654.4	sense	AGAGGCTGCCATCTACCACTTG	58	35	150
			antisense	CCAGACTTGGTGAGGACGATTA
PDH	PDHA1	NM_000284.3	sense	AAGAGAGGCGATTTCATTCCTG	56	35	248
			antisense	TTCACCATCCTGTCCTTGAGAA
PDK4	PDK4	NM_002612.3	sense	TCCAGACCAACCAATTCACATC	56	35	194
			antisense	GAACACCACCTCCTCTGTCTGA
FOXO1	FOXO1	NM_002015.3	sense	CCTGACCCAAGTGAAGACACCT	56	35	159
			antisense	ATGAACATGCCATCCAAGTCAC
FOXO3	FOXO3	NM_001455.3	sense	CTCTCTCTCAGGCTCCTCCTTG	56	35	215
			antisense	CCACGTTCAAACCAACAACATT
PPAR g	PPARG	NM_138712.3	sense	AGATGACAGCGACTTGGCAATA	56	35	207
			antisense	TCCGTGACAATCTGTCTGAGGT
PGC1 α	PPARGC1A	NM_013261.3	sense	GCCGTGTGATTTATGTCGGTAA	56	35	237
			antisense	GCTTGCGTCCACAAAAGTACAG
SIRT-1	SIRT1	NM_001142498.1	sense	GCAGATTAGTAGGCGGCTTGAT	56	35	153
			antisense	CTCTGGCATGTCCCACTATCAC
Mn-SOD	SOD2	X14322.1	sense	CTGCATCTGTTGGTGTCCAAG	56	35	160
			antisense	TAGTAAGCGTGCTCCCACACAT
HIF-1 α	HIF1A	NM_001530.3	sense	TACTCAAAGTCGGACAGCCTCA	54	35	179
			antisense	TTCGCTTTCTCTGAGCATTCTG
TNF-α	TNF	NM_000594.3	sense	CCATGTTGTAGCAAACCCTCAA	58	35	155
			antisense	CCTTGAAGAGGACCTGGGAGTA
18S	RNA18S1	M10098.1	sense	GTAACCCGTTGAACCCCATT	62	30	151
			antisense	CCATCCAATCGGTAGTAGCG

Table 1: Genes investigated and amplification data