Histological Feature Diet (n=5) Exercise (n=9) P Value DIWL vs EXτ
Pre Post P Value Pre Post P
Value
Steatosis              
% Hepatocytes affected
Score – no. of subjects
0 (<5%)
1 (5-33%)
2 (>33% - 66%)
3 (>66%)
No. pts with improvement - %
Pts with a reduction in score of ≥2* – no./total no. (%)
73 ± 36

0
1
0
4



23 ± 32

1
3
0
1
80%


4/5(80)
0.04



0.08





71 ± 32

0
2
1
6




54 ± 36

0
3
1
5
22%


1/9(11)
0.12  



0.59





0.05




0.02




Hepatocyte ballooning              
Score – no. of subjects
0 (None)
1 (Few)
2 (Many)
No. pts with improvement - %
2
2
1
4
1
0
4/5(80)
    0.50 1
4
4
3
3
3
4/9(44)
    0.34 0.06
0.30
Lobular inflammation              
Score – no. of subjects
0 (0 or 1 focus)
1 (2-4 foci per 200x field)
2 (>4 foci per 200x field)
No. pts with improvement - %
2
1
2
4
1
0
5/5(100)
    0.17 0
5
4
1
4
4
2/9(22)
    0.77 0.005
0.02
NAS              
Median (range)
Pts with a reduction in score of ≥2* – no./total no. (%)
5 (1-7) 2 (0-5)
4/5(80)
0.05 5 (2-8) 5 (1-7)
2/9(22)
0.29 0.02
0.09
Fibrosis              
Score – no. of subjects
0 (None)
1 (Perisinusoidal or periportal)
2 (Perisinusoidal and portal or periportal)
3 (Bridging fibrosis)
4 (Cirrhosis)
No. pts with improvement - %
Pts with a reduction in score of ≥2* – no./total no. (%)
2
2

1

0
0
4
1

0

0
0
4/5(80)
4/5(80)
0.50 3
2

2

2
0
5
1

0

1
2
6/9(67)
5/9(56)
1.0



0.13
1.0
0.58
NASH§              
Pts with resolution of NASH –no./total no. (%)   2/3(67)     2/8(25)   0.49
* Reduction in score of ≥2 or <2 but normalised tissue or was normal at baseline and remained normal
†p<0.05 RMANOVA for time × treatment difference between groups at 6 months
DIWL: Diet-Induced Weight Loss Intervention; EX: Exercise Intervention; NAS: NAFLD Activity Score; Pts: Patients; NASH: Non Alcoholic Steatohepatitis
§Number of patients with diagnosed NASH at baseline: n=3 in diet group and n=8 in exercise group
Table 2: Raw data of hepatic histologic scores pre and 6 months post-diet and exercise intervention in those patients with a repeat liver biopsy.