Figure 3: Insulin signaling in different tissues at 7 months after HFD feeding. Tissue samples from insulin clamp were subjected to the Western blot analysis detecting phosphorylation Ser473 AKT, Thr202/Tyr204 p44/42 MAPK (ERK1/2), and total proteins of each target. (A) gastrocnemius muscle, (B) liver, (C) heart, (D) GWAT. *p<0.04, n=4 per group.