Time points Baseline to HFD 5 months   HFD 5 to 7 months   Mouse strain WT mice Cpt1b+/- mice WT mice Cpt1b+/- mice Body weight > > Lean mass > = Fat mass > > Glucose tolerance ↓ = N/A N/A Insulin sensitivity ↓ = ↓ ↓↓ Insulin signaling integrity Skeletal muscle < > Liver < <   Heart < < Fat > > Lipid metabolites in skeletal muscle NEFA = = TAG = =   C4-OH acylcarnitine = < C18, C22:1-ceramide N/A <

Table 1: Summary of phenotypic changes during high fat diet-fed condition. =indicates similar trend, <indicates increased in Cpt1b+/- mice, >indicates decreased in Cpt1b+/- mice, ↓ indicates decreases during designated period in that strain.