Figure 5: β-cell mass in S6K2-/- mice.
A-F are representative images used to calculate β-cell mass from anti-insulin stained pancreas sections (brown) counterstained with hematoxylin (blue). A-D, the indicated strains were fed a HFD (scale bars indicate 3 mm) and inset images depict representative islets for each strain (scale bar 300 μm). E-F, the indicated strains were fed SD (scale bars indicate 4 mm) and inset images depict representative islets for each strain (scale bar 200 μm). β-cell mass was calculated as the percentage of insulin positive stained area relative to the total assessed pancreas area. Five to ten nonconsecutive sections separated by 200 μm were quantified for each pancreas (n=6-10 mice for each group). G, β-cell mass quantitated from images A-D. H, β-cell mass quantitated from images E-F.