Parameter C57BL/6J BALB/c A/J S6K2-/- Total Ketones, µmol/l 650.6 ± 89.0 738.2 ± 56.7 868.7 ± 115.3 743.2 ± 85.7 Insulin, ng/ml 3.0 ± 0.89 .048 ± 0.021 .123 ± 0.03 1.5 ± 0.30 Adiponectin, µg/ml 17.1 ± 0.86 14.7 ± 0.59 9.3 ± 0.31 15.1 ± 1.21 Glucose, mg/dl 300.0 ± 12.8 191.9 ± 6.1 200.7 ± 9.9 220.0 ± 12.2*** Cholesterol, mg/dl 247.1 ± 8.4 105.0 ± 3.9 110.0 ± 4.6 229.2 ± 26.8 LDH, U/L 938.6 ± 101.0 401.3 ± 48.1 367.1 ± 23.0 562.5 ± 61.9*** Triglycerides, mg/dl 53.6 ± 8.9 85.6 ± 7.7 94.3 ± 8.3 70.0 ± 8.2

Each value represents means ± S.E.M from groups of 8 mice

*** P<0.001 between C57BL/6 and S6K2-/- mice

Table 4: Serum chemistries of C57BL/6, BALB/c, A/J, and S6K2-/- mice at 51 weeks of age fed SD for 10 weeks followed by HFD for 41 weeks. Mice were fasted for 16 h prior to collection of serum.