Gene |
Single nucleotide Polymorphism |
PCR primers |
Restriction enzyme |
Fragment sizes |
Name |
Sequences (5’ to 3’) |
LMP2 (PSMB9) |
CGC/CAC codon 60 (R60H)[11,42] |
LMP2-2 |
GTGAACCGAGTGTTTGACAAGC |
Hha1 |
G:212, 40 bp |
|
rs17587 |
LMP2-1 |
GCCAGCAAGAGCCGAAACAAG |
37OC for 4 hours |
A: 252 bp |
LMP7 (PSMB8) |
CAG/AAG codon 49 (Q49K)[11,42] |
LMP7-Z |
TCGCTTTACCCCGGGGACTGa |
Pst1 |
C: 194, 18 bp |
|
rs2071543 |
LMP7-BR |
AACTTGCACTTCCTCCTCTCAGG |
37OC for 2 hours |
A:212 bp |
LMP7 (PSMB8) |
G/T intron 6 [11,25] |
LMP7-7 |
TTGATTGGCTTCCCGGTACTG |
Hha1 |
G: 428,180,155bp |
|
|
LMP7-4 |
TCTACTACGTGGATGAACATGG |
37OC for 5 hours |
T:583,180 bp |
* p value calculated using Fisher’s exact test
** Odds Ratio calculated using Woolf’s formula with Haldane’s modification
@ Haplotypes were made using SHEsis online software [19,20]
$ haplotypes showing significant differences are shown |