GENE SNP rs no FORWARD PRIMER REVERSE PRIMER FRAGMENT PCR PRODUCT ANNEALING TEMP ENZYME TCF7L2 INTRON 3 rs7903146 CCTAGTTAT CTGACATTGACT GAGAGCTA AGCACTTTTTAGGTA 188bp 60°C RsaI SLC30A8 EXON 8 rs13266634 GAAGTTGGA GTCAGAGCAGTC TGGCCTGTC AAATTTGGGAA 256bp 52°C HpaII

Table 2: List of Primer Sequence, Region Amplified, Amplicon Size, Detection Method and Annealing Temperature.