Figure 1: Nrf2 nuclear accumulation induced by NAFLD (Cellular distribution of Nrf2 obtained from the liver of mice treated with control, high-fat diet and pyrazole. An immunofluorescence assay was performed followed by detection with confocal microscopy. The cells were fixed, permeabilized and stained with antibodies against Nrf2. DAPI was used as a nuclear stain. Nrf2 specific nuclear staining by immunohistochemistry. Paraffin sections of livers of control, high-fat diet and pyrazole were stained using antibodies against Nrf2 followed by anti-rabbit horseradish peroxidase conjugate, as indicated. Chromagen development yields a brown color; sections were counter-stained with hematoxylin (blue). The increased brown staining of hepatocytic nuclei in upper panel as opposed to the more blue color of these nuclei in lower control panels indicated that Nrf2 was more abundant in high-fat diet and pyrazole nuclei. The image shows the results of a representative experiment from three independent experiments performed. Scale bars 20 mm).