Figure 3: NAFLD and pyrazole increase Nrf2 and Cyp2a5 protein expression in vivo (Western blot (A) and densitometric analysis (B) of Cyp2a5 and Nrf2 protein expression in liver obtained from mice treated with control, high-fat diet and pyrazole. Values represent the mean ± SD (n=3) expressed as a percent relative intensity of control. Values represent the mean ± SD (n=3). Values are significantly different from controls(** P<0.01). (C) COH activity was assessed in liver obtained from mice treated with control, high-fat diet and pyrazole. (D) Cyp2a5 and Nrf2 mRNA levels were quantified by RT-qPCR and normalized to GAPDH: average expression obtained from mice treated with control, high-fat diet and pyrazole. Values represent the mean ± SD (n=3). Values are significantly different from controls(** P<0.01)).