Figure 4: Cyp2a5 protein expression, mRNA levels and coumarin 7-hydroxylase activity in the Nrf2(-/-) mice (Western blot (A) and densitometric analysis (B) of Cyp2a5 and Nrf2 protein expression in liver obtained from mice treated with Control, high-fat diet and pyrazole. (C) Cyp2a5 mRNA levels were quantified by RT-qPCR and normalized to GAPDH. (D) COH activitywas assessed in liver obtained from mice treated with control, high-fat diet and pyrazole. Values represent the mean ± SD (n=3) expressed as a percent relative intensity of control).