Figure 5: Induction of GIP expression by nutrients. GIP mRNA in tissue and protein in supernatant were examined in cultured jejunum tissues of chow-fed mice after nutrient treatment for 2 hrs. A. GIP mRNA in the tissue. B. GIP peptide in the culture supernatant. The concentrations of nutrients are glucose (Glu, 20mM), cholesterol (CHO, 5mM), linoleic acid (300μM), palmitic acid (100μM), and BSA (3%). Data is presented as mean ± SE (n=6). *p< 0.05; **p< 0.001 vs. control by one-way ANOVA.