| GENES |
GENE POLYMORPHISM |
PRIMER SEQUENCE |
PCR
PRODUCT |
RESTRICTION ENZYMES |
FRAGMENT SIZE
WILD/MUT/HET |
PGC1 ALPHA
(rs8192678) |
Gly482Ser |
5’TATTTAGGGTTTTGCCAAGG3’
5’TTTGGAGGCAAGCAAGCAG3’
Annealing Temp: 58°C |
508bp |
Msp1 |
508/376,132/508,376,132 |
IRS1
(rs 1801278) |
G972R |
5’CGAGGTGTCCACGTAGC3’
5’CTTCTGTCAGGTGTCCATCC3’
Annealing Temp: 50°C |
260 bp |
BstN1 |
156,81,23/ 156,105,81,51,23/ 105,81,51,23 |
PI3 K
(rs 3730089) |
Met326Ile |
5’CCACAACGGTATGAATAACCATAT3’
5’CGAGATATCTCCCCAGTACC3’
Annealing Temp: 54°C |
65bp |
Nde1 |
43,22/43,22,65/65 |
KCNJ11
(rs 5219) |
E23K |
5’GACTCTGCAGTGAGGCCCTA3’
5’AGAAAAGGAAGGCAGACGAGAAG3’
Annealing Temp: 58°C |
218 bp |
BanII |
178,40/178,40,218/218 |
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