Figure 3: Gel permeation chromatography of urinary proteins from normal (NL) and diabetic (DM) rats. Urine samples (0.5 ml for normal, and 3 ml for diabeticrats) were concentrated to 0.4 ml, and applied to a Sephadex G-100 Superfine column (1 × 30 cm), equilibrated and eluted with phosphate buffer saline. Fractions (0.72 ml) were eluted at a constant flow rate (0.12 ml/min). Protein was measured by a modified Lowry method, with bicinchoninic acid (A562)(Brown RE, Jarvis KL, Hyland KJ (1989) Protein measurement using bicinchoninic acid: eliminationof interfering substances. Anal Biochem180: 136-139).