TLR4, NF-κB phosphorylation levels,

	Control		ONM		OHG		OTD
TLR4 antibody pretreatment	without	with	without	with	without	with	without	with
TLR4 mRNA/18s	1.03 ± 0.14	1.07 ± 0.13	1.43 ± 0.19^a	1.26 ± 0.14^a*	2.20 ± 0.25^ab	2.62 ± 0.20^ab*	2.15 ± 0.23^abc	1.44 ± 0.19^abc*
TLR4 Protein /β-actin	0.16 ± 0.05	0.15 ± 0.03	0.28 ± 0.06^a	0.17 ± 0.05^a*	0.40 ± 0.12^ab	0.38 ± 0.12^ab*	0.85 ± 0.08^abc	0.19 ± 0.05^abc*
NF-κB p65 protein/β-actin	0.16 ± 0.02	0.13 ± 0.03	0.26 ± 0.06^a	0.14 ± 0.03^a*	0.44 ± 0.05^ab	0.19 ± 0.04^ab*	0.86 ± 0.07^abc	0.21 ± 0.08^abc*
TNF-α(pg/ml)	225.79 ± 24.29	226.82 ± 26.72	253.30 ± 31.57^a	224.43 ± 26.71^a*	336.34 ± 26.55^ab	316.97 ± 35.75^ab*	456.79 ± 19.68^abc	412.87 ± 30.24^abc*
IL-1β(pg/ml)	135.96 ± 12.73	133.19 ± 16.15	253.30 ± 31.57^a	132.78 ± 23.67^a*	197.94 ± 16.13	183.51 ± 19.30^ab*	268.74 ± 14.50^abc	243.05 ± 19.72^abc*
ONM: obesity with normal metabolism; OHG: obesity with hyperglycemia; OTD: obesity with three kinds of metabolic disorders. ap<0.05 as compared with controls under the same pretreatment ; bp<0.05 as compared with ONM under the same pretreatment; cp<0.05 as compared with OHG under the same pretreatment; *p<0.05 as compared with the same group after 48h treatment.

Table 2: TLR4, NF-κB phosphorylation levels, TNF-α and IL-1β levels induced by the serum from obese in THP-1 cells preincubated with or without TLR4 monoclonal antibody.