Figure 4: The expression level of PDX-1 mRNA and protein in diabetes mice. (A): mice were treated with AS-IV or statin and the total RNA was extracted and RTPCR was used to detect the PDX-1 and GAPDH mRNA expression. The PDX-1/GAPDH ratios were expressed relative to the mean value of control. (B): Beta-cells were detected by PDX-1 staining in pancreatic islets obtained from the mice after STZ-administered and treated with saline or statin, or AS-IV, or statin and AS-IV. The quantification of insulin positive cells ratio were expressed relative to the mean value of control. Data are expressed as mean±S.E.M. *p < 0.05 versus saline group. The experiment was repeated three times.