RER VO2 (ml/g/h) VCO2 (ml/g/h) Heat /LBM (cals /h/g) Maximal aerobic capacity (ml O2/min/kg) Vehicle 0.776 ± 0.043 3038.01 ± 349.69 2366.75 ± 262.10 22 7 ± 0 78 50.3 ± 2.21 Compound 1 0.782 ± 0.038 3661.00 ± 760.21 2861.72 ± 542.77 25.9 ± 0.45* 57.4 ± 2.26*

Table 3: Compound 1 increases metabolic rate and energy expenditure in DIO mice Oxygen uptake (VO2), Carbon dioxide output (VCO2), respiratory exchange ratio (RER) and heat generation were measured by indirect calorimetry after 4 weeks of compound treatment in groups of 3 DIO mice from each group. Values were normalized to lean body mass (LBM), and represent means ± SD. Maximal aerobic capacity was calculated as milliliter (ml) O2/minute (min)/per gram (g) LBM. Statistical significance was calculated by using the two sample, paired t test. P values shown are for compound 1 versus vehicle group. *P=0.04 for heat production and P=0.02 for maximal aerobic capacity.